| 1 | 54.27 | Olicia WilliamsSt. Anthony's | St. Anthony's '12 | Saint Anthony's Invitational | 2011 |
| 2 | 54.31 | D'Jai BakerNorth Babylon | North Babylon '16 | New York State Track & Field Championships | 2014 |
| 3 | 54.43 | Elycia WilkersonConnetquot | Connetquot | Section XI State Qualifer Day 2 | 2026 |
| 4 | 55.13 | Erica YoungBellport | Bellport '04 | Section XI Individual Championships | 2004 |
| 5 | 55.67 | Brittany KorsahWest Babylon | West Babylon '17 | 46th Saint Anthony's Invitational | 2017 |
| 6 | 55.68 | Colby LubmanCommack | Commack '10 | Saint Anthony's Invitational | 2010 |
| 7 | 55.97 | Janet MellorMount Sinai | Mount Sinai '10 | SA Inv results 2 | |
| 8 | 56.24 | Alexa JacobsPort Jefferson | Port Jefferson '26 | Section XI State Qualifer Day 1 | 2026 |
| 9 | 56.29 | Kathleen CibulsBayport-Blue Point | Bayport-Blue Point '16 | Girls Division Championship Meet | 2014 |
| 10 | 56.3 | Michelle SeibertWard Melville | Ward Melville '05 | Derrick Adkins Holiday | 2005 |
| 11 | 56.39 | Maritza BlanchardMiddle Country | Middle Country '18 | Section XI State Qualifier Championships | 2018 |
| 12 | 56.45 | Amanda SteadMiller Place | Miller Place '17 | 46th Saint Anthony's Invitational | 2017 |
| 13 | 56.48 | Danielle RoweBay Shore | Bay Shore '12 | Stony Brook University - Wolfie Invitational | 2015 |
| 14 | 56.49 | Jessica GelibertBay Shore | Bay Shore '12 | Girls' Suffolk Coaches Meet | 2012 |
| 15 | 56.50 | Victoria LundinSt. John the Baptist | St. John the Baptist '11 | Girls Freshmen - Sophomore Championships | 2009 |
| 16 | 56.50 | Kerry OjedaMiddle Country | Middle Country '06 | Saint Anthony's Invitational | 2005 |
| 17 | 56.57 | Ryan WoolleySt. John the Baptist | St. John the Baptist '10 | Saint Anthony's Invitational | 2010 |
| 18 | 56.78 | Nadja AshleyWest Babylon | West Babylon '19 | Stony Brook Snowflake Classic | 2022 |
| 19 | 56.80 | Samara LawrenceNorth Babylon | North Babylon | Section XI State Qualifier | 2024 |
| 20 | 56.85 | Nia SingerBay Shore | Bay Shore '18 | Section XI State Qualifier Championships | 2018 |
| 21 | 56.88 | Jasmine DavisHauppauge | Hauppauge '15 | Massapequa Under the Lights | 2015 |
| 22 | 56.92 | Nicole HamiltonJohn Glenn | John Glenn '13 | Section XI Individual Championships | 2010 |
| 23 | 56.97 | Paige KeeferWest Babylon | West Babylon '17 | Saint Anthony's Invitational | 2016 |
| 24 | 57.18 | Leah HarrisCommack | Commack '29 | Section XI State Qualifer Day 2 | 2026 |
| 25 | 57.21 | Kaylee HammondStony Brook | Stony Brook | Stony Brook Snowflake Classic | 2022 |
| 26 | 57.27 | Sasa VannMercy | Mercy '12 | Girls Division Championship Meet | 2012 |
| 27 | 57.28 | Gianna PaulWalt Whitman | Walt Whitman '22 | Section XI State Qualifier Championships | 2018 |
| 28 | 57.38 | Kayla BermanWesthampton Beach | Westhampton Beach '18 | Section XI State Qualifier Championships | 2018 |
| 29 | 57.43 | Hayley CusackBayport-Blue Point | Bayport-Blue Point '04 | Section XI Individual Championships | 2004 |
| 30 | 57.44 | Analisse BatistaHuntington | Huntington '22 | The 18th Annual Bulldog Invite at Hewlett High School | 2022 |
| 31 | 57.45 | Jessica NischoSachem North | Sachem North '19 | Section XI State Qualifier Championships | 2018 |
| 32 | 57.49 | Mo LaClairCenter Moriches | Center Moriches '05 | Section XI Individual Championships | 2004 |
| 33 | 57.49 | Wilorda FrenelIslip | Islip '28 | Section XI State Qualifer | 2025 |
| 34 | 57.50 | Sofia ToepferCommack | Commack | Section XI State Qualifier | 2024 |
| 35 | 57.52 | Cameron CampoMount Sinai | Mount Sinai | Section XI State Qualifer | 2025 |
| 36 | 57.55 | Ariann RobinsonSachem East | Sachem East '22 | North Shore High School Invitational | 2022 |
| 37 | 57.55 | Natalie JasminBellport | Bellport '06 | Saint Anthony’s Invitational | |
| 38 | 57.59 | Donna JeantyBrentwood | Brentwood '10 | Saint Anthony's Invitational | 2010 |
| 39 | 57.64 | Kelly HughesMt. Sinai | Mt. Sinai | Joe Brandi Relays | 2025 |
| 40 | 57.66 | Melodee RileyRiverhead | Riverhead '12 | Girls Division Championship Meet | 2012 |
| 41 | 57.66 | Melissa JuneSt. Anthony's | St. Anthony's '14 | Suffolk Coaches Girls Track & Field Invitatio | 2014 |
| 42 | 57.68 | Madelyn SerxnerComsewogue | Comsewogue | Section XI State Qualifer | 2025 |
| 43 | 57.72 | Sarah MayeLongwood | Longwood '10 | Section XI Individual Championships | 2009 |
| 44 | 57.74 | Danielle SimonettiEast Islip | East Islip '26 | Section XI County 3A Championships 2024 | 2024 |
| 45 | 57.78 | Ana HerbertHarborfields | Harborfields '13 | Girls Division Championship Meet | 2012 |
| 46 | 57.84 | Sara RondeNorthport | Northport '12 | Girls Division Championship Meet | 2012 |
| 47 | 57.86 | Dana CerboneMiddle Country | Middle Country '18 | Stony Brook Snowflake Classic | 2023 |
| 48 | 57.90 | Brittany SheffeyBellport | Bellport '07 | Suffolk County Girls | 2004 |
| 49 | 57.91 | Sarah McCurdyBay Shore | Bay Shore '08 | Section XI Girls Team Championships | 2004 |
| 50 | 57.95 | Allyson GaedjeWard Melville | Ward Melville '18 | Westhampton Beach Invitational | 2018 |
| 51 | 57.97 | Mary ChimezieHalf Hollow Hills East | Half Hollow Hills East '16 | Stony Brook University - Wolfie Invitational | 2018 |
| 52 | 58.00 | Caprice ChurchAmityville | Amityville '04 | Section XI Girls Team Championships | 2004 |
| 53 | 58.00 | Oghenenyerovwo OmokeniStony Brook | Stony Brook | Stony Brook Snowflake Classic | 2023 |
| 54 | 58.01 | Jada HodgeMiddle Country | Middle Country '21 | Stony Brook Snowflake Classic | 2023 |
| 55 | 58.08 | Vinnelle GrantCopiague | Copiague '17 | Saint Anthony's Invitational | 2015 |
| 56 | 58.10 | Dana BegginsWest Babylon | West Babylon '17 | 46th Saint Anthony's Invitational | 2017 |
| 57 | 58.12 | Carissa DavisCentral Islip | Central Islip '09 | Section XI Individual Championships | 2008 |
| 58 | 58.12 | Melanie NotarstefaSachem East | Sachem East '12 | Section XI Individual Championships | 2011 |
| 59 | 58.14 | Emily TookerEastport-South Manor | Eastport-South Manor '10 | Girls Division Championship Meet | 2010 |
| 60 | 58.14 | Bridget CareySt. John the Baptist | St. John the Baptist '11 | NSCHSAA League Championships | 2011 |
| 61 | 58.16 | Lauren LoreficeWesthampton Beach | Westhampton Beach '15 | Saint Anthony's Invitational | 2015 |
| 62 | 58.23 | Emily FrostKings Park | Kings Park '09 | SA Inv results 2 | |
| 63 | 58.26 | Cypher McCloudCopiague | Copiague '12 | Section XI Individual Championships | 2011 |
| 64 | 58.26 | Brianna QuartararoComsewogue | Comsewogue '19 | 47th Saint Anthony's Invitational | 2018 |
| 65 | 58.26 | Madison MulderSmithtown West | Smithtown West '18 | Section XI State Qualifier Championships | 2018 |
| 66 | 58.30 | Kerri GraberHalf Hollow Hills West | Half Hollow Hills West '13 | Girls Division Championship Meet | 2012 |
| 67 | 58.30 | Michele DayeHalf Hollow Hills West | Half Hollow Hills West '20 | Suffolk Coaches Meet | 2019 |
| 68 | 58.35 | Maureen LaClairCenter Moriches | Center Moriches '05 | Section XI Girls Team Championships | 2004 |
| 69 | 58.39 | Amelia DeJoieSt. Anthony's | St. Anthony's | North Shore Invitational | 2025 |
| 70 | 58.40 | Kyra FranksMount Sinai | Mount Sinai '22 | Westhampton Track Invite Girls | 2022 |
| 71 | 58.42 | Melissa RibackWest Babylon | West Babylon '15 | Stony Brook University - Wolfie Invitational | 2018 |
| 72 | 58.42 | Sarafina ScavoSachem East | Sachem East | Joe Brandi Relays | 2025 |
| 73 | 58.51 | Jenna JacobsPort Jefferson | Port Jefferson '23 | East Hampton Invitational | 2023 |
| 74 | 58.52 | Megan FiedlerSt. Anthony's | St. Anthony's '13 | NSCHSAA League Championships | 2011 |
| 75 | 58.55 | Christina PerrierHalf Hollow Hills East | Half Hollow Hills East '14 | Girls Division Championship Meet | 2014 |
| 76 | 58.59 | Alyssa BarthelmesLindenhurst | Lindenhurst '13 | Girls Division Championship Meet | 2012 |
| 77 | 58.62 | Chelsea StokesMiller Place | Miller Place '09 | Section XI Individual Championships | 2009 |
| 78 | 58.64 | Aristea FranksMount Sinai | Mount Sinai '19 | Stony Brook Snowflake Classic | 2023 |
| 79 | 58.65 | Magdalena RobakHalf Hollow Hills West | Half Hollow Hills West '08 | SA Inv results 2 | |
| 80 | 58.67 | Sara AndradeBay Shore | Bay Shore '11 | Section XI Individual Championships | 2010 |
| 81 | 58.71 | Maddie LaezzaEastport-South Manor | Eastport-South Manor '27 | Section XI State Qualifer | 2025 |
| 82 | 58.73 | Shiseido RobinsonBrentwood | Brentwood '16 | Saint Anthony's Invitational | 2016 |
| 83 | 58.77 | Nickeela AustinCopiague | Copiague '16 | Girls Division Championship Meet | 2014 |
| 84 | 58.78 | Kelsey AmarosaSmithtown East | Smithtown East '12 | Section XI Individual Championships | 2011 |
| 85 | 58.78 | Shannon AhernKings Park | Kings Park '14 | Smithtown Invitational | 2014 |
| 86 | 58.80 | Arielle RossBellport | Bellport | Section XI State Qualifer Day 2 | 2026 |
| 87 | 58.81 | Kate DulmovitsSayville | Sayville '07 | Girls Division Championship Meet | 2005 |
| 88 | 58.82 | Laura LeeShoreham-Wading River | Shoreham-Wading River '13 | Girls Division Championship Meet | 2012 |
| 89 | 58.83 | Tenica RobertsBay Shore | Bay Shore '08 | Girls Division Championship Meet | 2008 |
| 90 | 58.83 | Priscilla St.LaurentBrentwood | Brentwood '06 | Saint Anthony’s Invitational | |
| 91 | 58.83 | Cameron CampoMt. Sinai | Mt. Sinai | Suffolk 4B County Championships | 2025 |
| 92 | 58.90 | Rebecca KellySjb | Sjb '07 | SA Inv results 2 | |
| 93 | 58.92 | Nikki MosbyLongwood | Longwood '13 | Girls Division Championship Meet | 2010 |
| 94 | 58.96 | Ryan WooleySt. John the Baptist | St. John the Baptist '10 | Saint Anthony's Invitational | 2009 |
| 95 | 58.98 | Sherika NicholasBellport | Bellport '07 | SA Inv results 2 | |
| 96 | 59.00 | Erica GoldmanSt. Anthony's | St. Anthony's '14 | NSCHSAA League Championships | 2014 |
| 97 | 59.02 | Christine DelianneWest Islip | West Islip '18 | 46th Saint Anthony's Invitational | 2017 |
| 98 | 59.04 | Cari RoccaroEast Islip | East Islip '12 | Girls Division Championship Meet | 2009 |
| 99 | 59.08 | Ashley WestEast Hampton | East Hampton '12 | Westhampton Beach Invitational | 2012 |
| 100 | 59.09 | Alena GannonNorth Babylon | North Babylon '13 | Girls Division Championship Meet | 2012 |
