| 1 | 26.99 | Kasey EstelleWest Islip | West Islip '09 | St. John's University Indoor Track Meet | 2010 |
| 2 | 27.28 | Cassie GibbonsBrentwood | Brentwood '14 | St Joseph's College Indoor Invitational | 2016 |
| 3 | 27.93 | Jessica FaustinMiddle Country | Middle Country '20 | Saint Anthony's Developmental Meet 3 | 2019 |
| 4 | 28.08 | Tiffany PerrierHalf Hollow Hills East | Half Hollow Hills East '13 | St. John's University Bill Ward Invitational | 2014 |
| 5 | 28.10 | Rebecca WrightLindenhurst | Lindenhurst '13 | East Coast Confrence Indoor Championships | 2014 |
| 6 | 28.13 | Nichole AndersonSt. Anthony's | St. Anthony's '14 | Saint Anthony's Girls Developmental Meet | 2013 |
| 7 | 28.30 | Tiese AntilusSt. John the Baptist | St. John the Baptist '19 | Saint Anthony's Developmental Meet 3 | 2019 |
| 8 | 28.32 | Laila GedeonSt. John the Baptist | St. John the Baptist '20 | Saint Anthony's Boys - Girls Developmental - Meet 3 | 2020 |
| 9 | 28.35 | Rachel RossLongwood | Longwood '09 | Molloy College Indoor Invitational | 2014 |
| 10 | 28.42 | Kathryn ManziStony Brook | Stony Brook | St. John's University Indoor Invitational | 2012 |
| 11 | 28.46 | Katie GallagherSt. Anthony's | St. Anthony's '11 | East Coast Confrence Indoor Championships | 2014 |
| 12 | 28.62 | Makayla SheltonSt. John the Baptist | St. John the Baptist '19 | Saint Anthony's Developmental Meet 3 | 2019 |
| 13 | 28.72 | Jessica PiernoBellport | Bellport '13 | St Joseph's College Indoor Invitational | 2016 |
| 14 | 28.80 | Casey HasherBayport-Blue Point | Bayport-Blue Point '13 | Molloy College Indoor Invitational | 2014 |
| 15 | 28.87 | Kristine CuartasBay Shore | Bay Shore '13 | St. John's University Bill Ward Invitational | 2015 |
| 16 | 28.94 | Kelsea SpencerSt. Anthony's | St. Anthony's '23 | Saint Anthony's Boys - Girls Developmental - Meet 3 | 2020 |
| 17 | 29.27 | Maria BasirSt. Anthony's | St. Anthony's '15 | Saint Anthony's Girls Developmental Meet 1 | 2011 |
| 18 | 29.33 | Gillian CutoloSt. John the Baptist | St. John the Baptist '15 | Saint Anthony's Girls Developmental Meet 1 | 2011 |
| 19 | 29.40 | Maggie ScottSt. John the Baptist | St. John the Baptist '19 | Saint Anthony's Developmental Meet 3 | 2019 |
| 20 | 29.46 | Jada AngelCopiague | Copiague '21 | Saint Anthony's Developmental Meet 3 | 2019 |
| 21 | 29.56 | Erin DonovanHalf Hollow Hills East | Half Hollow Hills East '12 | St. John's University Bill Ward Invitational | 2014 |
| 22 | 29.56 | Celeste CorcoranSt. John the Baptist | St. John the Baptist '14 | Saint Anthony's Girls Developmental Meet 1 | 2011 |
| 23 | 29.66 | Amanda ManninoSouthampton | Southampton '21 | Saint Anthony's Developmental Meet 3 | 2019 |
| 24 | 29.75 | Elizabeth BonafedeSt. Anthony's | St. Anthony's '15 | Saint Anthony's Girls Developmental Meet 1 | 2011 |
| 25 | 29.88 | Jennifer AngileriIslip | Islip '10 | Molloy College Indoor Invitational | 2014 |
| 26 | 29.96 | Gabrielle JeanSt. John the Baptist | St. John the Baptist '20 | Saint Anthony's Boys - Girls Developmental - Meet 3 | 2020 |
| 27 | 30.26 | Kristen LomantoRocky Point | Rocky Point '14 | Saint Anthony's Girls Developmental Meet | 2013 |
| 28 | 30.32 | Gabrielle MasonWesthampton Beach | Westhampton Beach '13 | St Joseph's College Indoor Invitational | 2018 |
| 29 | 30.47 | Amanda DolanSt. Anthony's | St. Anthony's '23 | Saint Anthony's Boys - Girls Developmental - Meet 3 | 2020 |
| 30 | 30.57 | Tess JablonowskiSt. Anthony's | St. Anthony's '22 | Saint Anthony's Boys - Girls Developmental - Meet 3 | 2020 |
| 31 | 30.57 | Toni BoltonSt. John the Baptist | St. John the Baptist '22 | Saint Anthony's Boys - Girls Developmental - Meet 3 | 2020 |
| 32 | 30.63 | Allison GrajalesSt. Anthony's | St. Anthony's '22 | Saint Anthony's Developmental Meet 3 | 2019 |
| 33 | 30.69 | Mallory DunphySt. John the Baptist | St. John the Baptist '19 | Saint Anthony's Developmental Meet 3 | 2019 |
| 34 | 30.93 | Kylie KoernerSachem East | Sachem East '21 | Saint Anthony's Developmental Meet 3 | 2019 |
| 35 | 30.94 | Nicole CalandraSt. John the Baptist | St. John the Baptist '19 | Saint Anthony's Developmental Meet 3 | 2019 |
| 36 | 30.96 | Caroline PalumboSouthampton | Southampton '19 | Saint Anthony's Developmental Meet 3 | 2019 |
| 37 | 30.97 | Lizzie BelmontePatchogue-Medford | Patchogue-Medford '17 | Saint Anthony's Girls Developmental Meet | 2013 |
| 38 | 31.02 | Emily GolubMiddle Country | Middle Country '22 | Saint Anthony's Developmental Meet 3 | 2019 |
| 39 | 31.07 | Caitlyn MastukaPatchogue-Medford | Patchogue-Medford '17 | Saint Anthony's Girls Developmental Meet | 2013 |
| 40 | 31.10 | Jadyn SimmonsSachem East | Sachem East '21 | Saint Anthony's Developmental Meet 3 | 2019 |
| 41 | 31.16 | Ashley AndersonSt. John the Baptist | St. John the Baptist '21 | Saint Anthony's Boys - Girls Developmental - Meet 3 | 2020 |
| 42 | 31.16 | Shannon GaffneySt. Anthony's | St. Anthony's '15 | Saint Anthony's Girls Developmental Meet 1 | 2011 |
| 43 | 31.25 | Mariella BilelloSt. Anthony's | St. Anthony's '12 | Saint Anthony's Girls Developmental Meet 1 | 2011 |
| 44 | 31.42 | Julianna KirshnerSt. Anthony's | St. Anthony's '23 | Saint Anthony's Boys - Girls Developmental - Meet 3 | 2020 |
| 45 | 31.56 | Kayla SchmadtkeWest Islip | West Islip '16 | Saint Anthony's Girls Developmental Meet | 2013 |
| 46 | 31.60 | Tamara WashingtonCopiague | Copiague '20 | Saint Anthony's Developmental Meet 3 | 2019 |
| 47 | 31.63 | Britney HuntMiddle Country | Middle Country '22 | Saint Anthony's Developmental Meet 3 | 2019 |
| 48 | 31.77 | Denisse AquinoPatchogue-Medford | Patchogue-Medford '14 | Saint Anthony's Girls Developmental Meet | 2013 |
| 49 | 31.80 | Kianna BurkeSt. Anthony's | St. Anthony's '22 | Saint Anthony's Boys - Girls Developmental - Meet 3 | 2020 |
| 50 | 31.81 | Tiffany LoachaminWest Babylon | West Babylon '17 | St. John's University 14th Annual Bill Ward Invitational | 2018 |
| 51 | 31.95 | Sophia CirielloJohn Glenn | John Glenn '20 | Saint Anthony's Developmental Meet 3 | 2019 |
| 52 | 32.18 | Elizabeth ThompsonSt. John the Baptist | St. John the Baptist '23 | Saint Anthony's Boys - Girls Developmental - Meet 3 | 2020 |
| 53 | 32.28 | Veronica LeddySt. Anthony's | St. Anthony's '12 | Saint Anthony's Girls Developmental Meet 1 | 2011 |
| 54 | 32.28 | Katherine FletcherSt. Anthony's | St. Anthony's '14 | Saint Anthony's Girls Developmental Meet 1 | 2011 |
| 55 | 32.41 | Kaitlyn QuinnSt. Anthony's | St. Anthony's '14 | Saint Anthony's Girls Developmental Meet 1 | 2011 |
| 56 | 32.42 | Isabelle RosadoSt. John the Baptist | St. John the Baptist '19 | Saint Anthony's Developmental Meet 3 | 2019 |
| 57 | 32.50 | Bailey WredeSt. John the Baptist | St. John the Baptist '21 | Saint Anthony's Boys - Girls Developmental - Meet 3 | 2020 |
| 58 | 32.58 | Sarah JohnsonBayport-Blue Point | Bayport-Blue Point | East Coast Confrence Indoor Championships | 2014 |
| 59 | 32.67 | Brie GallinSt. John the Baptist | St. John the Baptist '21 | Saint Anthony's Boys - Girls Developmental - Meet 3 | 2020 |
| 60 | 32.84 | Kelsey JordanWesthampton Beach | Westhampton Beach '17 | St Joseph's College Indoor Invitational | 2018 |
| 61 | 32.94 | Lauren ConnollyWalt Whitman | Walt Whitman '15 | St. John's University Bill Ward Invitational | 2015 |
| 62 | 33.07 | Moilly McGloneSt. John the Baptist | St. John the Baptist '22 | Saint Anthony's Boys - Girls Developmental - Meet 3 | 2020 |
| 63 | 33.11 | Genisis HerreraSt. John the Baptist | St. John the Baptist '22 | Saint Anthony's Boys - Girls Developmental - Meet 3 | 2020 |
| 64 | 33.21 | Maddie WymanJohn Glenn | John Glenn '22 | Saint Anthony's Developmental Meet 3 | 2019 |
| 65 | 33.66 | Olivia MenghiniPatchogue-Medford | Patchogue-Medford '14 | Saint Anthony's Girls Developmental Meet | 2013 |
| 66 | 33.95 | Margaret RothwellSt. Anthony's | St. Anthony's '15 | Saint Anthony's Girls Developmental Meet 1 | 2011 |
| 67 | 34.15 | Rebecca AndersonSt. Anthony's | St. Anthony's '14 | Saint Anthony's Girls Developmental Meet 1 | 2011 |
| 68 | 34.43 | Juliana SampugnaroSt. Anthony's | St. Anthony's | USATF Long Island Youth Indoor Track & Field Championships | 2024 |
| 69 | 34.61 | Emily FallonSt. Anthony's | St. Anthony's '15 | Saint Anthony's Girls Developmental Meet 1 | 2011 |
| 70 | 34.73 | Emily ParkerSt. John the Baptist | St. John the Baptist '21 | Saint Anthony's Boys - Girls Developmental - Meet 3 | 2020 |
| 71 | 34.89 | Isabelle PalumboSouthampton | Southampton '22 | Saint Anthony's Developmental Meet 3 | 2019 |
| 72 | 35.81 | Julia ImpagliazzoWest Islip | West Islip '17 | Saint Anthony's Girls Developmental Meet | 2013 |
| 73 | 37.83 | Nicole MejiaSt. Anthony's | St. Anthony's '14 | Saint Anthony's Girls Developmental Meet 1 | 2011 |
| 74 | 38.49 | Kendall GermainSt. Anthony's | St. Anthony's '23 | Saint Anthony's Boys - Girls Developmental - Meet 3 | 2020 |
